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Implementation of flow-through solid phase spectroscopic transduction with photochemically induced fluorescence: determination of thiamine [An article from: Analytica Chimica Acta]
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Implementation of flow-through solid phase spectroscopic transduction with photochemically induced fluorescence: determination of thiamine [An article from: Analytica Chimica Acta]
Implementation of flow-through solid phase spectroscopic transduction with photochemically induced fluorescence: determination of thiamine [An article from: Analytica Chimica Acta]

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Editorial Review: This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

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For the first time, flow-through solid phase spectroscopic transduction has been implemented with photochemically induced fluorescence. The system developed allows the sensitive, selective and rapid determination of thiamine in the presence of the rest of B-complex vitamins. By UV-irradiation in a single-line flow injection device, thiamine is converted on-line into a strongly fluorescent photoproduct, which is retained on C"1"8 silica gel filling the flow cell placed in the detector. The fluorescence of the photoproduct retained on C"1"8 is measured at excitation and emission wavelengths of 367 and 443nm, respectively. The sorption of the photoproduct on the solid support improves noticeabl...

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